Modifying Enzymes
Lifeline Cell Technology Trypsin 0.05% EDTA 0.02% (phenol red free), 100mL
Trypsin acts as an enzyme to help dissociate cells from their culture vessel. EDTA is a calcium chelator that helps to prevent clumping of cells. 0.05% Trypsin/0.02% EDTA Solution may be stored at -20°C until the expiration date or at 4-8°C for up to 1 month. This product is not approved for human or veterinary use or for use in in vitro diagnostics or clinical procedures.
Worthington Biochemical Corporation HEPATOCYTE ISOLATION
A component of the Hepatocyte and Papain Dissociation Systems. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease.
New England Biolabs, Inc. E.coli DNA Ligase – 200 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
Sigma Aldrich Fine Chemicals Biosciences β-Glucuronidase from limpets (Patella vulgata), 9001-45-0, MFCD00130629, 250,000 units
Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid, Synonym: β-D-Glucuronide glucuronosohydrolase.
Aldevron 2'O-Methyltransferase, 3400ug
3400ug dispense of 2'O-Methyltransferase. 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the m7Gppp5'N cap. 2'-O-Methyltransferase utilizes S-adenosylmethionine as the methyl group donor.
Genscript Corporation GenCrispr T7 Endonuclease I
T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA cruciform DNA structures Holliday structures or junctions hetero duplex DNA and more slowly nicked double-stranded DNA The cleavage site is at the first second or third phosphodiester bond that is 5 to the mismatch The protein is the product of T7 gene 3 GenCrispr T7 Endonuclease I is a fusion protein produced from i E coli /i
Zymo Research Corporation ZymoTaq™ PreMix (50 Rxns)
The inclusion of a heat-activated, thermal-stable DNA polymerase reduces primer dimer and non-specific product formation that can occur when performing conventional PCR. This unique product is specifically designed for the amplification of bisulfite-treated DNA for methylation detection, realtime and quantitative PCR that are SYBR Green and probe based. The ZymoTaq™ PreMix yields specific amplicon formation with little or no byproducts. Simple and easy to use, just add water, primers, and template DNA to the ZymoTaq™ PreMix and then heat at 95oC for 10 minutes to initiate polymerization.ZymoTaq™ DNA polymerase is a heat-activated, hot start polymerase that has 3-terminal transferase activity. The addition of A overhangs to amplified DNA makes it ideal for use in TA-cloning.
New England Biolabs, Inc. RNase Inhibitor, Murine (Glycerol-free) – 3000 units
RNase Inhibitor, Murine (Glycerol-free) is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). Recombinant murine RNase inhibitor lacks the cysteines identified in the human version to be sensitive to oxidation, which causes inactivation of the inhibitor. As a result, RNase Inhibitor, Murine has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, and is stable at low reducing agent concentrations (≤ 1 mM). This makes it ideal for reactions where low concentration reducing agent is required (e.g. Real-time RT-PCR).
Sigma Aldrich Fine Chemicals Biosciences Lipase from Aspergillus Oryzae | 9001-62-1 | MFCD00131509 | 250ml
Lipase from Aspergillus Oryzae | 9001-62-1 | MFCD00131509 | 250ml
Sigma Aldrich Fine Chemicals Biosciences Deoxyribonuclease I from bovine pancreas | 9003-98-9 | MFCD00130918 | 20 KU
Deoxyribonuclease I from bovine pancreas | Mol Wt: 31 kDa | 9003-98-9 | MFCD00130918 | 20 KU
Cayman Chemical Heptnoyl-Coenzyme AsodIum 1mg
A medium-chain acyl CoA; inhibitsbutyryl-CoA:acetate-CoA transferase isolated from P. elsdenii at 200 µM; levels are increased in primary fibroblasts derived from patients with MCAD deficiency in the presence of heptanoic acid; blocks berberine-induced decreases in infarct volume and improvements in motor function in a mouse model of MCAO-induced ischemic stroke at 0.4 mg/kg per day
New England Biolabs, Inc. DNase I-XT-1000 units
An engineered variant of DNase I DNase I-XT is a salt-tolerant DNA endonuclease that nonspecifically cleaves DNA to release di- tri- and oligonucleotide products with 5 prime phosphorylated and 3 prime hydroxylated ends. DNase I-XT acts on single- and double-stranded DNA chromatin and the DNA strand of RNADNA hybrids. While DNase I (M0303) is inhibited by salt concentrations greater than 50 mM DNase I-XT exhibits optimal activity between 50-100 mM salt and retains 65% and about 40% activity in 200 and 300 mM salt respectively. This increased salt tolerance makes DNase I-XT the preferred enzyme for DNA template removal from an in vitro transcription (IVT) reaction. Importantly DNase I-XT is RNase-free allowing for the complete removal of DNA from RNA preparations while maintaining RNA integrity.
New England Biolabs, Inc. EnGen Spy Cas9 HF1 - 2500 pmol
EnGen Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from Streptococcus pyogenes with reduced non-specific DNA cleavage. Spy Cas9 is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The single guide RNA (sgRNA) targets Cas9 to the region immediately upstream of a 5'-NGG-3' protospacer adjacent motif (PAM) producing a double stranded break 3 bases upstream of the PAM. EnGen Spy Cas9 HF1 contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C-termini of the protein.
Sigma Aldrich Fine Chemicals Biosciences Carbonic Anhydrase from bovine erythrocytes | 9001-03-0 | MFCD00081467 | 100 mg
Carbonic Anhydrase from bovine erythrocytes | Purity: ≥95% | 9001-03-0 | MFCD00081467 | 100 mg
New England Biolabs, Inc. Uracil-DNA Glycosylase (UDG) – 5000 units
E. coli Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).