Modifying Enzymes
Discovery Life Sciences HUMAN CYP19 + P450 REDUCTASE S
Human CYP19 (Aromatase) + Reductase, BD Gentest;
Worthington Biochemical Corporation PDS KIT 1 VIAL
Papain Vial, Papain Dissociation System, A Vial Reconstituted with 5mL of EBSS or Equivalent Yields a Solution at 20units of papain/mL in 1mM L-cysteine with 0.5mM EDTA, Store at 2 deg. to 8 deg.C, Source: Papaya Latex, 1 Vial
Bio IVT Mouse CYP3A11 Low Reductase Bactosomes, 1nmol/vial
Mouse CYP3A11 Low Reductase Bactosomes, 1nmol/vial
New England Biolabs, Inc. ZraI – 1000 units
A restriction endonuclease that recognizes the sequence GAC_^GTC.
Gold Biotechnology Inc IPTG 500 g
IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.
New England Biolabs, Inc. USER® Enzyme – 250 units
USER (Uracil-Specific Excision Reagent) Enzyme generates a single nucleotide gap at the location of a uracil. USER Enzyme is a mixture of Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease VIII. UDG catalyses the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease VIII breaks the phosphodiester backbone at the 3 and 5 sides of the abasic site so that base-free deoxyribose is released.Applications of USER include:
- Directional RNA Seq
- NEBNext adaptor cleavage
- USER cloning
New England Biolabs, Inc. E.coli DNA Ligase – 200 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
New England Biolabs, Inc. ZraI – 200 units
A restriction endonuclease that recognizes the sequence GAC_^GTC.
New England Biolabs, Inc. TelN Protelomerase – 250 units
TelN Protelomerase, from phage N15, cuts dsDNA at a TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage.
New England Biolabs, Inc. SacI-HF – 2000 units
A high fidelity restriction endonuclease that recognizes the sequence G_AGCT^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. NotI-HF® – 500 units
A high fidelity restriction endonuclease that recognizes the sequence GC^GGCC_GC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
- Reduced star activity
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
New England Biolabs, Inc. α1-2,4,6 Fucosidase O - 400 units
a1-2,4,6 Fucosidase O is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal a1-2, a1-4 and a1-6 linked fucose residues from oligosaccharides. a1-2,4,6 Fucosidase O cleaves a1-6 fucose residues more efficiently than other linkages.
LGC GENOMICS LLC (Lucigen) NxGen M-MuLV Reverse Transcriptase, 50,000 UOutstanding cDNA synthesis.
Supplied at 200,000 U/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25 C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25 C. 250,000 U is supplied as 5 50,000 U.
Sigma Aldrich Fine Chemicals Biosciences Neuraminidase (Sialidase) from Clostridium perfringens |
Neuraminidase (Sialidase) from Clostridium perfringens |
New England Biolabs, Inc. GpC Methyltransferase (M.CviPI) – 1000 units
Methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5´...GC...3´.